Blake L, Watkins L, Liu Y, Inoue T, Wu B. A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies. Nat Comm, 2024, DOI: 10.1038/s41467-024-46943-z
Latallo M*, Wang S*, Dong D*, Nelson B, Livingston N, Wu R, Zhao N, Stasevich T, Bassik M, Sun S†, Wu B†, Single molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTD, Nat Comm, 2023, DOI: 10.1038/s41467-023-41339-x, (*equal contribution, †co-corresponding author)
Livingston N.M., Kwon J., Valera O., Saba J.A., Sinha N.K., Reddy P., Nelson N., Wolfe C., Ha T., Green R., Liu J., Wu B. Bursting translation on single mRNAs in live cells. Molecular Cell, 2023 June 16; 83:1–14. doi: https://doi.org/10.1016/j.molcel.2023.05.019.
Blake L, De La Cruz A, Wu B, Imaging spatiotemporal translation regulation in vivo, Seminars in Cell & Dev Bio, 2023, DOI: 10.1016/j.semcdb.2023.03.006
Imoto Y., Raychaudhuri S., Ma Y., Fenske P., Sandoval E., Itoh K., Blumrich E., Matsubayashi H., Mamer L., Zarebidaki F., Söhl-Kielczynski B., Trimbuch T., Nayak S., iwasa J., Liu J., Wu B., Ha T., Inoue T., Jorgensen E., Cousin M., Rosenmund C., Wantanabe S. Dynamin is primed at endocytic sites for ultrafast endocytosis. Neuron, 2022 Jul 8;110(17):2815-2835.e13. DOI: 10.1016/j.neuron.2022.06.010
Huimin Yu, Z Zhang, G Li, Y Feng, L Xian, F Bakhsh, D Xu, C Xu, T Vong, Wu B, F M. Selaru, F Wan, M Donowitz, GW. Wong, Adipokine C1q/Tumor necrosis factor- Related Protein 3 (CTRP3) Attenuates Intestinal Inflammation via Sirtuin 1 / NF-κB Signaling, Cellular and Molecular Gastroenterology and Hepatology, 2022, https://doi.org/10.1016/j.jcmgh.2022.12.013.
Wang S*, Latallo M.J.*, Zhang Z, Huang B, Bobrovnikov D, Dong D, Livingston N.M., Tjoeng W, Hayes L.R., Rothstein J.D., Ostrow L.W., Wu B‡, Sun S‡. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD. Nature Communications, 2021 Aug 13; 12(1):4908. doi: https://doi.org/10.1038/s41467-021-25082-9. PubMed PMID: 34389711.
Zou R.S.*, Liu Y*, Wu B, Ha T. Cas9 deactivation with photocleavable guide RNAs. Molecular Cell, 2021, Apr 1; 81:1-13. doi: https://doi.org/10.1016/j.molcel.2021.02.007. PubMed PMID: 33662274.
Goldman D.H., Livingston N.M., Movsik J, Wu B‡, Green R‡. Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling. Molecular Cell, 2021, Apr 15; 81:1-11. doi: https://doi.org/10.1016/j.molcel.2021.01.029. PubMed PMID: 33581075.
Liu Y*, Zou RS*, He S, Nihongaki Y, Li X, Shiva Razavi S, Wu B‡, Ha T‡. Very fast CRISPR on demand. Science, 2020, Jun 12;368(6496): 1265-1269. doi: 10.1126/science.aay8204. PubMed PMID: 32527834.
Blake LA, Wu B. One Message, Many Translations: Heterogeneity Revealed with Multicolor Imaging. Molecular Cell, 2019, Jul 11;75(1):3-4. doi: 10.1016/j.molcel.2019.06.026. PubMed PMID: 31299206.
Sato H, Wu B, Delahaye F, Singer RH, Greally JM. Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains. J Cell Biol, 2019, Jun 3;218(6):1810-1823. doi: 10.1083/jcb.201811109. Epub 2019, May 20. PubMed PMID: 31110057; PubMed Central PMCID: PMC6548134.
Latallo M.J.*, Livingston N.M.*, and Wu B. Translation imaging of single mRNAs in established cell lines and primary cultured neurons, Methods, 2019, DOI: https://doi.org/10.1016/j.ymeth.2019.03.021
Adivarahan S, Livingston N.M., Nicholson B, Rahman S, Wu B, Rissland O.S., and Zenklusen D, Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway, Molecular Cell, 2018, Vol 72, 727–738, DOI: https://doi.org/10.1016/j.molcel.2018.10.010
Tutucci E, Livingston N.M., Singer R.H., and Wu B. Imaging mRNA In Vivo, from Birth to Death, Annual Review of Biophysics, 2018, Vol 47: 85-106, DOI: https://doi.org/10.1146/annurev-biophys-070317-033037
Biswas J, Liu Y, Singer R.H., and Wu B. Fluorescence Imaging Methods to Investigate Translation in Single Cells, Cold Spring Harbor Perspectives in Biology, 2018, DOI:10.1101/cshperspect.a032722
Pre-Hopkins lab
Yoon Y.J.*, Bin Wu*, Buxbaum A.R., Sulagna D, Tsai A, English B.P., Grimm J.B., Lavis D.L. and Singer R.H., Glutamate-induced RNA localization and translation in neurons, Proceedings of National Academy of Science, 2016, DOI:10.1073/pnas.1614267113, (*equal contribution)
Bin Wu, Eliscovich C, Yoon Y and Singer R.H., Translation dynamics of single mRNAs in live cells and neurons, Science, Vol 352, Issue 6292, 2016, pp. 1430-1435, DOI:10.1126/science.aaf1084
Bin Wu, Buxbaum A.R., Katz Z, Yoon, Y and Singer R.H, Quantifying protein-mRNA interactions in single live cells, Cell, 2015, 162 (1): 211-220, PMID 26140598 / PMCID: PMC4491145
Bin Wu*, Miskolci, V*, Sato H., Tutucci E., Kenworthy C.A., Donnelly S.K., Yoon Y.J., Cox, D, Singer R.H. and Hodgson L., Synonymous modification results in high fidelity gene expression of repetitive protein and nucleotide sequences, Genes & Developments, 2015, 29 (8): 876-886, PMID: 25877922 / PMCID: PMC4403262 (*equal contribution)
Bin Wu, Chen, J., Singer R.H., Background free imaging of single mRNAs in live cells using split fluorescent proteins, Scientific Reports, 2014, 4:3615
Bin Wu, Singer, R.H., Mueller J.D., Time-integrated fluorescence cumulant analysis and its application in living cells, Methods in Enzymology, 2013; 518:99-119, PMID:23276537/PMCID: PMC4152916
Bin Wu, Chao J. A., Singer, R.H., Fluorescence Fluctuation Spectroscopy Enables Quantitative Imaging of Single mRNAs in Living Cells, Bioph J. 2012, 102, 2936-2944, PMID: 22735544 / PMCID: PMC3379624
Bin Wu*, Piatkevich K*, Lionnet T, Singer R, Verkhusha V,Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization and dynamics, Current Opinion in Cell Biology, 2011, 23, 1-8, PMID: 21242078 / PMCID: PMC3143818, (*equal contribution)
Bin Wu, Chen Y., Mueller JD. Heterospecies partition analysis reveals binding curve and stoichiometry of protein interactions in living cells, Proceedings of National Academy of Science, 2010, 107, 4117-4122, PMID: 20142515 / PMCID: PMC2840129
Bin Wu, Chen Y., Mueller JD. Fluorescence fluctuation spectroscopy of mCherry in living cells, Biophys. J. 2009: 96 2391-2404, PMID: 19289064 / PMCID: PMC2907682
Bin Wu, Chen Y., Mueller JD. Fluorescence correlation spectroscopy of finite-size particles, Biophys. J 2008: 94, 2800-2808, PMID: 18065475 / PMCID: PMC2267132
Bin Wu, Chen Y., Mueller JD. Dual-color time-integrated fluorescence cumulant analysis, Biophys. J. 2006: 91 2687-2698, PMID: 16815900 / PMCID: PMC1562389
Bin Wu, Mueller JD. Time-integrated fluorescence cumulant analysis in fluorescence fluctuation spectroscopy, Biophys. J. 2005: 89 2721-2735, PMID: 16055549 / PMCID: 1366773
Buxbaum, A.R., Wu, B, Singer R.H., Single β-Actin mRNA detection in neurons reveals a mechanism for regulating its translatability, Science, 2014, 343 (6169): 419-422, PMID:24458642 / PMCID: PMC4121734
Larson D, Zenklusen D, Wu B, Singer R, Real time observation of transcription initiation and elongation on an endogenous gene, Science, 2011, 332, no. 6028, 475-478: PMID: 21512033 / PMCID: PMC3379624
